Action of the in vitro antifungal activity of Copaifera officinalis, Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis and Thymus vulgaris essential oils on causative agents of onychomycosis
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Abstract
Onychomycosis is defined as a fungal disease characterized by the infectious process of the region ungueal and is responsible for triggering a poor quality of life for patients and although considered superficial mycoses, are difficult to diagnose and treat, thus has driven the conducting research in an attempt to obtain other therapeutic options in addition to synthetic drugs available in healthcare. Given the above, the present study aimed to evaluate the in vitro action of the essential oils of Copaifera officinalis, Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis and Thymus vulgaris against fungi that cause onychomycosis. The in vitro antifungal activity of the selected essential oils was investigated using the hole agar plate test method against Candida albicans (ATCC 10231), Microsporum canis (ATCC 14055) and Trichophyton rubrum (ATCC 28189) strains 100%, 25% and 5% and the reading of the plates and interpretation of the results evaluated from the observation of the inhibition halo, considering the area without growth detectable to the naked eye. Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis and Thymus vulgaris essential oils showed antifungal activity with halo formation on the yeast and dermatophytes fungal species, and Copaifera officinalis oil showed only a weak activity in relation to fungal species tested. Therefore, it is concluded that the analyzed essential oils presented antifungal activity, opening the perspective of further studies to characterize their antimicrobial principles and natural alternatives for treating this disease.
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