Microbicidal effect of ozone gas on Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans
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Abstract
Objective: To evaluate the microbicidal effect of ozone gas at different concentrations and times before pathogenic microorganisms: Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans grown in Petri dishes. Material and Methods: The effect of ozone gas against standardized strains of the bacteria Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923 and the yeast Candida albicans ATCC 10231 was tested in six different concentrations using an ozone generator. Group 1: without treatment; experimental groups with treatment: ozone: Group 2: 60 µg / ml at 10 minutes, Group 3: 40 µg / ml at 10 minutes, Group 4: 30 µg / ml at 10 minutes, Group 5: 20 µg / ml at 5 minutes and Group 6: 15 µg / ml at 5 minutes. The microorganisms were inoculated into Petri dishes with blood agar and then received the ozone gas. Results: Ozone gas showed antimicrobial activity in most of the concentrations used. Conclusion: Ozone gas showed a valuable antimicrobial activity against the microorganisms tested. A single topical spray application of each used ozone concentration inhibited the growth of most strains of potentially pathogenic bacteria with known resistance to antimicrobial agents.
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References
Andreula C. Ozone therapy. Neuroradiology. 53 (Suppl 1): S207–S209 - DOI 10.1007/s00234-011-0930-7
Sousa C. S., Torres L. M., Azevedo M. P. F., Camargo T. C., Graziano K. U., Lacerda R. A., Turrini R. N. T. Ozônio na esterilização de produtos para assistência à saúde: revisão integrativa da literatura. Rev Esc Enferm USP 2011; 45(5):1243-9.
Schaechter M., At al, Microbiologia – Mecanismos das Doenças Infecciosas, Editora Guanabara, 2002 , 3° ed.
ZAIT, Clarisse, at al – Micologia Médica, Editoria MEDISI, 1998, Rio de Janeiro
Sidrim J. J.C, Rocha M. F. G, Micologia Médica À Luz de Autores Contemporâneos, Editora Guaabara, 2004.
LEVINSON W., Microbiologia Médica e Imonologia, Editora Artmed, 2010, 10° ed, Porto Alegrre- RS
ABOZ, Ozonioterapia – Relatório Técnico, 2014/2013, São Paulo
Declaração de Madri, Ozonioterapia, 2ª ed. , 2015, Madrid (Espanha)
Fontes at Al, Effect of low-dose gaseous ozone on pathogenic bactéria, Bio Med Central. 2012.
Zhang at al. Effect of ozone on membrane permeability and ultrastructure in Pseudomonas aeruginosa, Jornal of Applied Microbiology, 2011.